海洋生物技术原理和套用

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海洋生物技术原理和套用【海洋生物技术原理和套用】《海洋生物技术原理和套用》是1997年海洋出版社出版的图书，作者是张士璀、马军英、范晓，。
基本介绍书名：海洋生物技术原理和套用
作者：张士璀、马军英、范晓
ISBN：7-5027-4225-5
页数：294
出版社：海洋出版社
出版时间：1997-01-01
装帧：平装
图书目录1 绪论...... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ( 1)1.1 何谓海洋生物技术........................... ................................................... ( 1)1.2 海洋生物技术研究现状........................................................................ ( 2)2 海洋微生物的筛选和培养........................................................................... ( 6)2.1 概论... ... ... ...... ............... ... ... ......... ... ... ............ ... ... ... ............ ... ... ...... ( 6)2.2 海洋微生物的採样、分离和培养技术...................................................... ( 7)2.3 细菌的分类、鉴定与保存................................................................ . .(13)2.4 产生EPA的海洋细菌的筛选............................................................... (19)2.5 油细菌的筛选.................................................................................... (20)2.6 海洋蓝藻的採集、分离和培养............................................................... (22)3 海洋动物细胞的培养.................. ......... ... ... ... ...... ...... ... ... ... ... ...... ...... ... ... ... (28)3.1 概论.................................................................. ......................... . .(28 )3.2 鳗鲡、鲤鱼肝细胞的培养................................................................ . .(28)3.3 鳗鲡肝细胞灌流法的操作................................................................ . .(29)3.4 分离鳗鲡肝细胞的原代单层培养法......................................................... (34)3.5 振荡法分离鲤鱼肝细胞................................................................... . .(35)3.6 分离鲤鱼肝细胞的原代单层培养............................................................ (35)3.7 培养肝细胞的实验........................ .................. ............................... . .(3 6)3.8 草鱼肾组织细胞的原代培养和传代培养实验............................................. (39)4 大型海藻的组织培养............................................................................ . .(4 1)4 .1 概论. . ... ......... ... ... ... ... ......... ... ... ... ... ... ... ... ... ... .. ... ... ... ... ... ... ... . . .(41 )4.2 组织培养的设备和基本技术............................................................. . .(43)4.3 外植体的无菌化技术...................................................................... . .(48)4.4 海藻组织块愈伤组织的诱导............................................. .................... (49)4.5 无菌培养系的愈伤组织诱导.................................................................. (50)4.6 细胞培养.......................................................................................... (51)5 海洋动物的细胞操作........................ ......................................................... (56)5.1 概论... ................................................... .......................................... (56) 5.2 性决定(性别控制)..................... ......................................................... (59)5.3 嵌合体动物的培育.............................. ................................................ (62)6 大型海藻的细胞操作............ ... ... ... ......... ... ... ............................ ... ......... ... ... (65)6.1 概论................................................................................................ (65)6.2 大型海藻的培养................................................................................. (68)6.3 原生质体分离技术......................................................................... . .(70)6.4 原生质体培养技术......................................................................... . .(74)6.5 细胞融合技术............................................................................... . .(77)6.6 异种融合细胞的筛选及个体再生............................................................ (82)7 海洋微藻的细胞操作... ... ............... ... ... ......... ... ... ............ ... ... ............ ......... (85)7.1 概论............................................. ........................... ... ..................... (85)7.2 海洋微藻的分离和纯化技术.................................................................. (88)7.3 培养基.................................... ......................................................... (91)7.4 海洋微藻的生长测量和培养技术............................................................ (96) 7.5 原生质体分离技术........................................................................... (100)7.6 原生质体培养技术.................. ......................................................... (103)7.7 细胞融合技术................................................................................. (104)7.8 细胞学技术................................................................................ .(105)8 海产鱼类的染色体操作........................................................................... (109)8.1 概论... ................................................... ....................................... (109)8.2 天然多倍体......... ...... ............ ... ......... ... ... ... ... ...... ... ... ... ............ ...... (109)8.3 人工诱导的多倍体...... ..................................................................... (111)8.4 单性发育................................................................................... .(118)8.5 染色体片断移植.............................................................................. (123)8.6 染色体操体技术的套用及其发展趋势......................................... . . . .(123)9 海产无脊椎动物的染色体操作......... ......................................................... (129)9.1 概论... ............ ... ... ............ ...... ...... ... ... ... ......... ... ... ............ ... ......... (129)9.2 细胞核及染色体的观察方法............................................................... (132) 9.3 人工多倍体诱导法........................................................................... (135)9.4 多倍体的鉴定................................................................................. (137)10 海洋动物的基因克隆.............................................................................. (141)10.1 概论... ... ... ... ... ... ... ... ...... ... ... ... ......... ... ... ... ... ... ... ......... ... ... ...... ...... (141)10.2 海洋动物的cDNA 克隆.................................................................. (143)10.3 目的cDNA克隆的鉴定.................................................................. (166)10.4 DNA 序列测定.............................................................................. (181)11 转基因海洋动物........................ ............................................................ (190)11.1 概论..................................................................... ........................ (190 )11.2 基因导入方法................................................................................. (195)11.3 转基因动物的鉴别...................................................................... . . (198)12 海洋微藻的基因工程...... ........................................................................ (200)12.1 概论......... ... ... ... ...... ...... ... ... ............ ... ... ............ ... ... ... ............... ... (200)12.2 载体的开发............................................................... ..................... (201) 12.3 受体系统.......................................................... ....................... . . (202)12.4 外源基因转移方法........................................................................... (203)13 大型海藻的基因工程................................. ......... . . ............................. (208)13.1 概论............................................................................................. (208)13.2 材料的选择.................................................................................... (210)13.3 大型海藻的核酸製备............................................. ........................... (210)13.4 大型海藻的目的基因................................................................... . . (211)14 纯种保存... ... ... ... ... ... ... ... ... ... ... ...... ... ......... ... ... ... ... ... ... ... ... ... ... ... ... ... ... (213)14.1 概论............................................................................................. (213)14.2 大型藻类的纯种保存........................................................................ (215)14.3 海洋微型生物的冷冻保存.................................................................. (217)14.4 海洋植物的冷冻保存........................................................................ (220)14.5 海洋动物生殖细胞的冷冻保存............................................................ (222)15 生物反应器.......................................................................................... (225) 15.1 概论............................................................................................. (225)15.2 微生物固定化......... ............................................................ ............ (226)15.3 利用生物反应器生产有用物质............................................................ (228)15.4 海洋蓝藻生物反应器........................................................................ (231)16 生物感测器... ... ......... ... ... ... ... ......... ... ... .................. ............ ... ... ... ......... (235)16.1 概论............................................................................................. (235)16.2 接受器的固定化.............................................................................. (237)16.3 换能器.......................................................................................... (239)16 .4 套用... . .. . .. .. . . . . . . . .. . .. . . . .. . .. . . .. . .. .. . . .. . .. . . . . . . .. . .. . . .. . . . .. . . .. . . . .. . . .. . . . (24 1)16.5 结语.............................................................................. ............... (243)17 海洋环境保护............................................................... ........................ (245)17.1 概论............................................................................................. (245)17.2 石油的生物降解.............................................................................. (247) 17.3 卤代烃的生物降解........................................................................... (248)17.4 酶工程方法消除工业废水中的酚与芳香胺............................................. (250)17.5 藻类对重金属离子的吸附.................................................................. (253)17.6 可降解塑胶................................................................................ .(255)17.7 生物技术在解决生物耗氧化合物对环境污染方面的套用........................... (256)18 疾病的诊断与防治................................................................................. (257)18.1 概论............................................................................................. (257)18.2 诊断........................... .................................................................. (258)18.3 防治............................................................................................. (260)19 海洋生物活性物质... ... ... ... ... ... ... ... ... ...... ... ...... ... ...... ... ... ... ... ... ... ...... ... ... (261)19. 1 概论... ... ... ... ... ... ... ... ... ... .... . ....... ... ... ... ... ...... ... ... ... ... ... ... ...... .. .(261)19.2 海洋生物活性物质的研究方法........................................................ .(267)20 海洋新材料— —甲壳质....................................................................... .(271)20.1 概论... ... ...... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ...... ... ... ... ... ... ... ... ... ... ... ... (271) 20.2 人工皮肤............ ........................................................................... (272)